## Abstract Free polyamines in rat tissues were determined by high performance capillary electrophoresis using fluorescence detection. After precipitation of proteins with perchloric acid, the sample solution was derivatized with fluorescamine using a microscale procedure. Ethylenediamine was added
β¦ LIBER β¦
Effect of polyamines on the separation of ovalbumin glycoforms by capillary electrophoresis
β Scribed by Maria Estrella Legaz; Mercedes M. Pedrosa
- Book ID
- 107785650
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 780 KB
- Volume
- 719
- Category
- Article
- ISSN
- 1873-3778
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The oligosaccharides in ovalbumin as a glycoprotein model were released with anhydrous hydrazine, and reductively pyridylaminated after re-N-acetylation. The derivatives were analyzed by capillary zone electrophoresis (CZE) with on-column fluorometric detection. Direct CZE could separate the derivat