Effect of polyamines on cyclic AMP-dependent and independent protein kinases from mouse epidermis
β Scribed by Andrew W. Murray; Mario Froscio; Anne Rogers
- Book ID
- 118310868
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 318 KB
- Volume
- 71
- Category
- Article
- ISSN
- 0006-291X
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## Abstract Cyclic AMP is one of the factors which regulate cell differentiation. In animals the apparent mechanism of action of cyclic AMP is the activation of protein kinase enzymes. Postnatal changes in cyclic AMPβdependent protein kinase enzymes were therefore examined. Determinations were made
Extracts obtained from rat hepatocytes incubated with saline, glucagon or insulin were electrophoresed on polyacrylamide gels and then assayed for cyclic (3H)AMP binding capacity. Analysis of the binding patterns demonstrated that glucagon dissociated a holoenzyme of cyclic AMP-dependent protein kin