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Effect of ouabain on the meiotic maturation of stage IV-VXenopus laevis oocytes

✍ Scribed by Penna, Mary Jo ;Wasserman, William J.

Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 754 KB
Volume: 241
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402410108

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✦ Synopsis

Full-grown stage VI Xenopus laevis oocytes (1,200 to 1,300pm) respond to progesterone stimulation by undergoing a series of physiological and morphological changes that are referred to as meiotic maturation. Oocytes in earlier stages of oogenesis (I through V) do not undergo these changes and remain in prophase arrest when exposed to this steroid. We have found that oocytes ranging from 850 pm (stage IV) to 1,000 pm (stage V) are capable of responding to progesterone under the appropriate conditions. Oocytes 2 850 pm in diameter underwent germinal vesicle breakdown (GVBD) after 10-12 hr of exposure to progesterone when ouabain was added to the medium at a concentration > 2.5 x 1OP6M. Under this culture condition, progesterone was

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