Initiation of meiotic maturation inXenopus laevis oocytes by the combination of divalent cations and ionophore A23187
✍ Scribed by Wasserman, William J. ;Masui, Yoshio
- Publisher
- John Wiley and Sons
- Year
- 1975
- Tongue
- English
- Weight
- 278 KB
- Volume
- 193
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Abstract
Meiotic maturation was induced in Xenopus laevis oocytes when the external Ca^++^ or Mg^++^ion concentration was raised above 5 mM in the presence of the ionophore. Ionophore‐divalent cation‐induced maturation appears to be due to the stimulation of the oocyte itself. Cytoplasm of responding oocytes induced maturation when microinjected into ovarian oocytes. Cycloheximid, an inhibitor of progesterone‐induced maturation, inhibited the maturational response induced by the ionophore and divalentications. Ethidium bromide, an inhibitor of the follicular response to human chorionic gonadotropin, had no effect. The possible roles that Ca^++^ and Mg^++^ may play in the initiation of maturation are discussed.