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Effect of osmotic stress on glucose oxidase production and secretion by Aspergillus niger

✍ Scribed by J. Fiedurek

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
102 KB
Volume
38
Category
Article
ISSN
0233-111X

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✦ Synopsis

A mycelium of Aspergillus niger was cultured under various osmotic stresses. When fungus was cultured under osmotic stress, total catalase activity increased with increasing concentration of NaCl, up 0.4 M, but glucose oxidase under this condition significantly decreased. Mycelial growth was repressed with increased media osmolarity. To release periplasmic glucose oxidase 72-h old mycelium was suspended on a concentrated solution of NaCl (0.4 -2.8 M/l). The highest yield of GOD activity was obtained at a NaCl concentration of 1.2 M at pH 6.0, which improved the activity of this enzyme by about 2.1-fold in comparison with the control medium without this depressor.

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