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Effect of nourseothricin (streptothricin) on the outer membrane of sensitive and resistant Escherichia coli strains

✍ Scribed by Dr. sc. nat. Guntram Seltmann; Ernst-Jürgen Wolter

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
453 KB
Volume
27
Category
Article
ISSN
0233-111X

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✦ Synopsis

Nourseothricin (streptothricin) causes disturbances (perforations) in the outer membrane of sensitive E. coli strains allowing lysozyme and deoxycholate, but not the periplasmic alkaline phosphatase to penetrate. EDTA slightly increases, but Mg++ ions slightly decrease this effect. The cell walls of three from four nourseothricin-resistant strains do not become permeable under these conditions, but remain sensitive against TRIS/EDTA. Nourseothricin is supposed to pass the outer membrane of sensitive bacteria via some kind of "self-promoting" pathway. This way can (but need not) be blocked in resistant strains.

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