The outer membranes of Escherichia coli and Salmonella typhimurium contain several proteins that have been shown to produce rather large channels for diffusion [ 11 . One class, called "porins," has apparent molecular weights in the range of 34-37,000, and, when reconstituted into phospholipid-lipo
Anchorage of cyclodextrin glucanotransferase on the outer membrane of Escherichia coli
β Scribed by Hsiao-Ming Wan; Ban-Yang Chang; Sung-Chyr Lin
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 135 KB
- Volume
- 79
- Category
- Article
- ISSN
- 0006-3592
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A rapid and simple method for separating and isolating the inner and outer membranes of Escherichia coli is described. Membrane vesicles were prepared either by passing the bacteria through a French press or by conversion of the cells to spheroplasts by the lysozyme-EDTA treatment and disruption of