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Effect of mode of operation of bioreactors on the biosynthesis of penicillin amidase in Escherichia coli

✍ Scribed by K. Jagannadha Rao; T. Panda

Publisher: Springer
Year: 1996
Tongue: English
Weight: 439 KB
Volume: 14
Category: Article
ISSN: 1615-7605
DOI: 10.1007/bf00369476

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✦ Synopsis

Different operational mode of bioreactors influence the biosynthesis of the enzyme and related products as well as the growth of industrial microorganisms. This communication deals with the effect of mode of operation of various bioreactors with different geometric configurations, viz., batch (includes commercially available batch stirred tank, and custom-designed cylindrical and tapered reactors), batch-fed, continuous flow stirred tank reactors on the biosynthesis of penicillin amidase in Escherichia coil Experimental findings show that the biosynthesis of penicillin amidase in E. coli show a little variation among batch reactor modes and significant variation on the continuous mode of operation. Further analysis show that the different reactor modes also influence periplasmic localization of the enzyme in the cell.

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