Effect of metformin on insulin binding to receptors in cultured human lymphocytes and cancer cells
โ Scribed by V. Pezzino; V. Trischitta; F. Purrello; R. Vigneri
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 495 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0012-186X
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In the present study, we investigated the mechanism by which the antidiabetic drug phenformin increases insulin binding to its receptors in IM-9 human cultured lymphocytes. After a 24-hr preincubation, phenformin induced a twofold increase in specific '251-insuIin binding, and removal of phenformin
The effect of metformin on hepatocyte insulin receptor binding was examined in normal, streptozotocin diabetic and genetically obese diabetic (ob/ob) mice. In normal mice, chronic administration of metformin (60 mg X kg-1 X day-1 for 50 weeks) increased the number of low affinity receptors by 148%.
Insulin has both short- and long-term effects on cellular metabolism. The short-term effects are known to involve the insulin receptor, a protein kinase capable of phosphorylating itself and other proteins. The role of the receptor was elucidated by studies of a mutant insulin receptor which lacked