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Effect of lysine residues on the deamidation reaction of asparagine side chains

✍ Scribed by Sante Capasso; Gianfranco Balboni; Paola Di Cerbo

Publisher
Wiley (John Wiley & Sons)
Year
2000
Tongue
English
Weight
98 KB
Volume
53
Category
Article
ISSN
0006-3525

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✦ Synopsis

The effect of lysine residues on the deamidation reaction of the asparagine side chain has been studied on the peptide Խ Խ Ac-Cys-Lys-Asn-Gly-Gln-Thr-Asn-Cys-NH 2 and on its lysine-acetylated derivative in a wide range of pH values. The amino acid sequence of these peptides is similar to the local sequence flanking the labile Asn-67 in RNAse A. The experimental data show that Lys influences both the deamidation rate and the relative yield of the two reaction products, i.e., the aspartic acid and ␀-aspartic acid containing peptide. These effects are pH dependent and can be rationalized based on the mechanism previously proposed for the deamidation reaction via succinimide derivative.

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