Effect of low culture temperature on specific productivity, transcription level, and heterogeneity of erythropoietin in Chinese hamster ovary cells
โ Scribed by Sung Kwan Yoon; Ji Yong Song; Gyun Min Lee
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 163 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0006-3592
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## Abstract To investigate the effect of culture temperature on erythropoietin (EPO) production and glycosylation in recombinant Chinese hamster ovary (CHO) cells, we cultivated CHO cells using a perfusion bioreactor. Cells were cultivated at 37ยฐC until viable cell concentration reached 1โรโ10^7^ c
When three recombinant Chinese hamster ovary (rCHO) cell lines, CHO/dhfr-B22-4, CS13-1.00\*, and CS13-0.02\*, were cultivated in hyperosmolar media resulting from NaCl addition, their specific foreign protein productivity increased with medium osmolality. However, due to a simultaneous suppression o