During transformation of mustard seedlings cotyledons from storage organs to photosynthetically competent leaves, a process which occurs during the first 4 d after sowing, total glutamine-synthetase (GS, EC 6.3.1.2) activity increases from zero to the high level usually observed in green leaves. In
Effect of light on the development of glyoxysomal functions in the cotyledons of mustard (Sinapis albaL.) seedlings
β Scribed by D. Bajracharya; P. Schopfer
- Book ID
- 104750905
- Publisher
- Springer-Verlag
- Year
- 1979
- Tongue
- English
- Weight
- 941 KB
- Volume
- 145
- Category
- Article
- ISSN
- 0032-0935
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β¦ Synopsis
The degradation of storage fat in the cotyledons of mustard seedlings is unaffected by phytochrome and photosynthesis (irradiation with continuous red or far-red light from sowing of the seeds) although light imposes a strong constraint on the translocation of organic matter from the cotyledons into the seedling axis. Likewise, the development and disappearance of glyoxysomal enzyme activities (isocitrate lyase, malate synthase, citrate synthase) takes place independently of light. It is concluded that the mobilization of storage fat (fatβcarbohydrate transformation) is independent of photomorphogenesis. The surplus of carbohydrate produced from fat in the light seems to be converted to starch grains in the plastids, which function as a secondary storage pool in the cotyledons.
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Density-labelling with deuterium oxide has been used to distinguish preexisting from newly made ascorbate oxidase (EC 1.10.3.3) molecules in cotyledons of the mustard seedling (Sinapis alba L.). The time course of the change in bandwidth of isopycnically banded enzyme, taken together with the accomp
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