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Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase-like phytase, PhyAsr

✍ Scribed by Robert J. Gruninger; L. Brent Selinger; Steven C. Mosimann


Book ID
111312509
Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
548 KB
Volume
275
Category
Article
ISSN
1432-1327

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The oxidation state of the cysteine residue at the active site of human protein tyrosine phosphatase (PTP-1B) greatly affects its enzymatic activity. We wished to examine peroxide-treated preparations for modifications of this enzyme with electrospray mass spectrometry in order to determine the loca