Effect of hypoxia on the superficially exposed carbohydrates of subcellular fractions of guinea pig cerebral cortex

✍ Scribed by Magdalena Rossowska

• Publisher: John Wiley and Sons
• Year: 1982
• Tongue: English
• Weight: 465 KB
• Volume: 7
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.490070109

✦ Synopsis

Abstract

Experimental hypoxia led to changes in carbohydrate‐containing macromolecules located on the microsomal and synaptosomal membrane surface. It was found that hypoxia reduced the external membrane‐bound sialic acid content in microsomal and synaptosomal fractions.

On the other hand, a decrease in fucose content of proteins in hypoxic synaptosomal fraction was found. Similarly, a decrease was observed in the amount of ^3^H‐labeled concanavalin A bound to the microsomal and synaptosomal glycoprotein fractions. Treatment of microsomal and synaptosomal fractions with galactose oxidase followed by reduction with tritiated potassium borohydride resulted in a significantly higher incorporation rate of tritium into the hypoxic membranes. This was accompanied by an increase in the endogenous glycoprotein galactose transferase activity both in microsomal and synaptosomal fractions.