Effect of human lymphoblastoid interferon on insulin synthesis and secretion in isolated human pancreatic islets

✍ Scribed by C. J. Rhodes; K. W. Taylor

• Publisher: Springer
• Year: 1984
• Tongue: English
• Weight: 280 KB
• Volume: 27
• Category: Article
• ISSN: 0012-186X
• DOI: 10.1007/bf00276977

✦ Synopsis

Human islets of Langerhans were isolated from the pancreas removed from a 13-year-old female transplant donor. The islets were incubated in a culture medium for 24 h in the presence of human lymphoblastoid interferon (1000 units/ml). Insulin secretion, proinsulin biosynthesis, total protein biosynthesis and total insulin content were assessed at various concentrations of glucose in the presence of interferon. In interferon-treated islets glucose-stimulated insulin secretion was unaltered from that of control islets; however, glucose-stimulated proinsulin biosynthesis was specifically inhibited by interferon (48%, p less than 0.025). Total protein biosynthesis and total insulin content were not significantly affected by interferon.