The properties and cellular distribution of a high-affinity uptake mechanism for taurine have been investigated using separate populations of purified chick embryo neural retina neurons and glia. Purified neuronal monolayers, cultured in scrumfree medium. were incubated in radioactive taurine under
Effect of hepes on the taurine uptake by cultured glial cells
β Scribed by P. L. Lleu; G. Rebel
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 754 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0360-4012
No coin nor oath required. For personal study only.
β¦ Synopsis
HEPES inihibited the taurine uptake in glial cells. A different kind of inhibition was observed when HEPES was present in the culture medium or in the incubation medium used for the taurine-uptake measurement. As an example of a possible interference of HEPES in pharmacological experiments, we have studied the effect of this buffer on the modulation of taurine uptake by p agonists or ionic concentration.
π SIMILAR VOLUMES
Guanidinoethane sulfonate (GES) markedly decreased 3H-taurine accumulated in the retina by the high-affinity uptake process. The effect of GES was dosedependent. Analysis of the kinetics of GES effect revealed that it is a competitive inhibitor. The uptake of taurine by GES was less affected in rat
The uptake of [45Ca] has been studied in clonal glial and neuronal cells. It was somewhat more efficient in the neuroblastoma clone MI compared to glial clones. In all cases [45Ca] uptake was shown to depend on the phosphate concentration in the incubation medium. It was decreased by the ionophore A
Labelled histamine was taken up into cultured glial cells of chick embryonic brain by a system with high affinity for histamine and diffusion. The active uptake, occurring at low concentrations of the amine, was Na+ dependent and gave an apparent K, of 0.24 pM and a V, , , of 0.31 pmol x mg protein
Growth of KB cells was inhibited by both spermine and spermidine, but the inhibition is reduced in conditioned medium. The amount of spermine required for 50% inhibition of plating varied according to the type of serum used with medium 199 (calf, fetal bovine, and horse; 0.55, 0.9, and 24 pg/ml resp