Effect of heavy water on three high producing strains of Penicillium chrysogenum

✍ Scribed by Kasheed Mohammed; Daniel A. Nona; Martin I. Blake

• Publisher: John Wiley and Sons
• Year: 1970
• Tongue: English
• Weight: 366 KB
• Volume: 59
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600590335

✦ Synopsis

Three high antibiotic-producing strains of Penicillium chrysogenum (Wisconsin Q176, were examined for their ability to grow and produce penicillin in nutrient solutions containing pure heavy water. Penicillin titer values and pH changes were observed over the culture study period. Wisconsin Q176 responded most favorably. Continuous feeding of dextrose and potassium phenylacetate gave better antibiotic titers than a single addition of the two components. With either feeding regimen, potassium phenylacetate was more effective as a precursor than N-(p-hydroxyethy1)phenylacetamide or a-phenylacetamide. L-Leucine added together with potassium phenylacetate further enhanced penicillin production. The production of the yellow pigment associated with Wisconsin Q176 in protio nutrient cultures was suppressed in deuterium oxide nutrient cultures.

Keyphrases 0 Deuterium oxide effect-Penicilhm chrysogenum penicillin production [7 Penicillin production, P. chrysogenumdeuterium oxide effect 0 Media additives, effect-penicillin production Additives, single, multiple feedings-penicillin production, P. chrysogenum ( 5 ) F. V. Soltero and M.