✦ LIBER ✦

Energetics of growth and penicillin production in a high-producing strain of Penicillium chrysogenum

✍ Scribed by W. M. vanGulik; M. R. Antoniewicz; W. T. A. M. deLaat; J. L. Vinke; J. J. Heijnen

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 127 KB
Volume: 72
Category: Article
ISSN: 0006-3592
DOI: 10.1002/1097-0290(20000120)72:2<185::aid-bit7>3.0.co;2-m

No coin nor oath required. For personal study only.

✦ Synopsis

The results of a large number of carbon-limited chemostat cultures of Penicillium chrysogenum carried out on glucose, ethanol, and acetate as the growth limiting substrate have been used to obtain an estimation of the adenosine triphosphate (ATP) costs for mycelium growth, penicillin production, and maintenance and the overall stoichiometry of oxidative phosphorylation of the fungus. It was found that penicillin production was accompanied by a significant additional energy drain (73 mol of ATP per mole of penicillin-G) from primary metabolism. This finding has been confirmed in independent experiments and has been shown to result in a significantly lower estimate for the maximum theoretical yield of penicillin-G on the carbon source.

📜 SIMILAR VOLUMES

Alginate oligosaccharides as enhancers o

Alginate oligosaccharides as enhancers of penicillin production in cultures of penicillium chrysogenum

✍ Bolatito T. Ariyo; Christopher Bucke; Tajalli Keshaverz 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 119 KB 👁 2 views

Oligosaccharide fragments were prepared by partial acid hydrolysis of sodium alginate and consisted of oligomannuronate (OM) and oligoguluronate (OG) blocks. Effects of the OM and OG blocks on penicillin G production by P. chrysogenum were investigated. The oligosaccharides were found to cause signi

Penicillin productivity and glutathione-

Penicillin productivity and glutathione-dependent detoxification of phenylacetic and phenoxyacetic acids in Penicillium chrysogenum

✍ Tamás Emri; Éva Leiter; Etelka Farkas; István Pócsi 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 93 KB 👁 1 views

Pathway kinetics and metabolic control a

Pathway kinetics and metabolic control analysis of a high-yielding strain of Penicillium chrysogenum during fed batch cultivations

✍ Pedro de Noronha Pissara; Jens Nielsen; Michael J. Bazin 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 31 KB 👁 2 views

Production of penicillin in a fluidized-

Production of penicillin in a fluidized-bed bioreactor: Control of cell growth and penicillin production by phosphate limitation

✍ Deok K. Oh; Chang K. Hyun; Jung H. Kim; Young H. Park 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 445 KB 👁 1 views

Continuous cultivations of a Penicillium

Continuous cultivations of a Penicillium chrysogenum strain expressing the expandase gene from Streptomyces clavuligerus: Growth yields and morphological characterization

✍ J. Robin; G. Lettier; M. McIntyre; H. Noorman; J. Nielsen 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 191 KB 👁 1 views

Dependence of Penicillium chrysogenum gr

Dependence of Penicillium chrysogenum growth, morphology, vacuolation, and productivity in fed-batch fermentations on impeller type and agitation intensity

✍ P. Jüsten; G. C. Paul; A. W. Nienow; C. R. Thomas 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 300 KB 👁 2 views

The influence of the agitation conditions on the growth, morphology, vacuolation, and productivity of Penicillium chrysogenum has been examined in 6 L fedbatch fermentations. A standard Rushton turbine, a fourbladed paddle, and a six-bladed pitched blade impeller were compared. Power inputs per unit