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Effect of growth conditions on the rate of loss of the plasmid pAT153 from continuous cultures of Escherichia coli HB101

✍ Scribed by Lawrence C.K. Chew; William C.A. Tacon; Jeffrey A. Cole


Book ID
109314516
Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
303 KB
Volume
56
Category
Article
ISSN
0378-1097

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Insertion of foreign DNA into the unique HindIII site of the high copy number plasmid pAT153 reduces but does not completely abolish the resistance of Escherichia coli HB101 cells to tetracycline. Recombinant DNA-containing colonies could then be phenotypically differentiated from non-recombinant on