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Effect of glycerol on thermolysin-catalyzed peptide bond synthesis

โœ Scribed by Ian Durrant; Robert J. Beynon; Paul B. Rodgers

Book ID
115707230
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
527 KB
Volume
250
Category
Article
ISSN
0003-9861

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Effect of water and enzyme concentration
Effect of water and enzyme concentration on thermolysin-catalyzed solid-to-solid peptide synthesis
โœ Markus Erbeldinger; Xiongwei Ni; Peter J. Halling ๐Ÿ“‚ Article ๐Ÿ“… 1998 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 88 KB

We have studied a thermolysin-catalyzed solidto-solid dipeptide synthesis using equimolar amounts of Z-Gln-OH and H-Leu-NH 2 as model substrates. The high substrate concentrations make this an effective alternative to enzymatic peptide synthesis in organic solvents. Water content was varied in the r