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Effect of genotype on somatic embryogenesis from immature cotyledons of soybean

✍ Scribed by W. A. Parrott; E. G. Williams; D. F. Hildebrand; G. B. Collins

Publisher
Springer
Year
1989
Tongue
English
Weight
328 KB
Volume
16
Category
Article
ISSN
0167-6857

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✦ Synopsis

Genotype has a large effect on the ability of immature soybean cotyledons to undergo auxin-stimulated somatic embryogenesis. Among 33 soybean lines, all those showing good regeneration were found to have in their pedigrees one or both of the highly regenerative ancestral lines, 'Manchu' or 'A.K. Harrow'. When 'Manchu' was crossed with 'Shiro', a genotype showing extremely poor regeneration, F~ hybrid cotyledons showed intermediate regeneration capacity. This paper is published with the approval of the director of the Kentucky Agricultural Experiment Station.

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Immature soybean (Glycine max (L.) Merr) embryos, or cotyledons isolated from them, were cultured on modified MS medium containing B5 vitamins and NAA (50 #M) to induce somatic embryogenesis. The effects of media variables, dissection treatments and light conditions were investigated in this system.