✦ LIBER ✦

Effect of ester moiety of substrates on enantioselectivity of protease catalysis in organic media

✍ Scribed by Katsuhiro Kawashiro; Hideki Sugahara; Tomoya Tsukioka; Shigeru Sugiyama; Hiromu Hayashi

Publisher: Springer Netherlands
Year: 1996
Tongue: English
Weight: 417 KB
Volume: 18
Category: Article
ISSN: 0141-5492
DOI: 10.1007/bf00129339

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✦ Synopsis

The a-chymotrypsin-catalyzed transesterification between a racemic N-trifluoroacetylphenylalanine ester and 1-propanol, was carried out in organic media. Although activation of the substrate by introducing electron-withdrawing group to the ester moiety enhanced the rate of reaction, it decreased enantioselectivity at the same time. In the instance of subtilisin Carlsberg, inversion of the L-specificity was observed.

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