The present study was designed to examine the effects of media support on the frequency of embryo and plant production from cultured anthers of soft-red winter wheat. Approximately twice as many embryos were produced when anthers were cultured in a liquid as compared to an agar-solidified medium. Up
Effect of embryo age and culture media on plant growth and vernalization response in winter wheat
β Scribed by H. C. Sharma; B. S. Gill
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 407 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0014-2336
No coin nor oath required. For personal study only.
β¦ Synopsis
Embryo age and composition of nutrient medium affected plant growth and response to vernalization in winter wheat (Triticum aestivum L.). Root and shoot development was more in older than in younger excised embryos, and more in a medium without kinetin than in one with kinetin. Kinetin (2 mg/l) in the medium did not accelerate vernalization, probably because it tended to inhibit seedling and plant growth. Embryo age and media did not completely replace vernalization. Twenty-and 16-day-old embryos responded by flowering after 4 weeks of vernalization. Among plants raised on a standard medium from Z&day-old embryos and vernalized for 4 weeks, 84.29: flowered by or before 50 days after transplanting. Time from embryo culture to heading for 2(tday-old embryos with 4-week vernalization averaged 84.6 days. Immature embryos (I 6-20 days old) needed only 4 weeks of vernalization compared to 6 weeks for mature embryos.
Excised embryos could be vernalized as efficiently as seedlings raised by embryo culture. Embryo culture at 1620 days after anthesis coupled with 4-week cold treatment shortens generation time of winter wheat by about 40 days.
' Contribution
No. 82-131-j,
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