Effects of support medium on embryo and plant production from cultured anthers of soft-red winter wheat (Triticum aestivumL.)
โ Scribed by A. M. Jones; J. F. Petolino
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 390 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0167-6857
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โฆ Synopsis
The present study was designed to examine the effects of media support on the frequency of embryo and plant production from cultured anthers of soft-red winter wheat. Approximately twice as many embryos were produced when anthers were cultured in a liquid as compared to an agar-solidified medium. Upon transfer to regeneration medium, a significantly lower percentage of the embryos produced in liquid regenerated plants. The addition of activated charcoal to an agar-solidified medium resulted in a considerable increase in embryo production, however, plant regeneration from embryos produced on charcoalcontaining medium was significantly lower than those produced on agar only. Embryo production frequencies ranged from 2.4-13.2 and 2.5-32.2 embryos per 100 anthers on media with and without charcoal, respectively. Plant regeneration frequencies from embryos produced in the presence of activated charcoal ranged from 0-5.5% as compared to 0-39.1% from embryos produced in the absence of charcoal. More than twice as many embryos produced on Ficoll-containing liquid medium regenerated plants when compared to embryos produced in liquid only. The results from this study suggest that cultural modifications designed to maximize embryo production must take into account the quality of the resulting embryos as they relate to plant regeneration.
๐ SIMILAR VOLUMES
Four winter wheat (Triticum aestivum L.) and two spring wheat cultivars were evaluated in anther culture on three to four different media for their ability to initiate callus and green plants. Five media were used in the experiment: stored-potato medium with Ficoll 400, fresh-potato medium with Fico