Effect of Dopamine on Viability of BHK-21 Cells
β Scribed by D. A. Moshkov; M. B. Abramova; V. S. Shubina; V. P. Lavrovskaya; L. L. Pavlik; E. I. Lezhnev
- Book ID
- 106358709
- Publisher
- Springer US
- Year
- 2010
- Tongue
- English
- Weight
- 430 KB
- Volume
- 149
- Category
- Article
- ISSN
- 0007-4888
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Removal of serum from BHK-21/C13 cells in culture results in a decline in thymidine incorporation extending over five days. Additional re- moval of any of several amino acids results in a rapid decrease in incorporation of thymidine to negligible levels by 24 hours. Replacement by complete medium th
B H K 21/13 cells, 50 gcnerutioris f r o m cloning, are rransplaiitablc~ owing to the prcwiice qf a sniall proportion (about 0.00i 74) of' highly tuniorigenic variarir cells. Noti-tuiriorigeriic cells can easily be i.solated,froni thcsr sfoclis by srcloning. With passagc in vitro the proportion of