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Effect of amino acid deprivation on DNA synthesis in BHK-21/C13 cells

✍ Scribed by William T. Melvin; Julian F. Burke; Alison A. Slater; Hamish M. Keir

Publisher: John Wiley and Sons
Year: 1979
Tongue: English
Weight: 534 KB
Volume: 98
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040980109

No coin nor oath required. For personal study only.

✦ Synopsis

Removal of serum from BHK-21/C13 cells in culture results in a decline in thymidine incorporation extending over five days. Additional re- moval of any of several amino acids results in a rapid decrease in incorporation of thymidine to negligible levels by 24 hours. Replacement by complete medium then provokes a synchronous wave of DNA synthesis after only ten hours with DNA synthesis first increased a t six hours. Starvation for glutamine results in a rapid decline in protein synthesis over the 24 hour period when DNA synthesis is falling. However, there is considerable degradation of total protein during this period, and RNA degradation is also greatly increased. Concurrently, synthesis of RNA falls to less than 10% of that in control cells. '62) were grown in monolayer culture a t 37°C

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