Effect of diffusional limitations on lineweaver-burk plots for immobilized enzymes

✍ Scribed by Bruce K. Hamilton; Colin R. Gardner; Clark K. Colton

• Publisher: American Institute of Chemical Engineers
• Year: 1974
• Tongue: English
• Weight: 796 KB
• Volume: 20
• Category: Article
• ISSN: 0001-1541
• DOI: 10.1002/aic.690200310

✦ Synopsis

Abstract

Lineweaver‐Burk plots of reaction rate data obtained with immobilized enzymes need not be linear even when intrinsic enzyme kinetics follow the simple Michaelis‐Menten rate expression. Theoretical calculations show that mass transfer effects may cause curvature which is concave or convex to the abscissa, depending upon experimental conditions. Consequently, graphical procedures commonly employed for analysis of soluble enzyme kinetics may yield misleading results when applied to immobilized enzymes. Three approaches which follow from the behavior of numerical and asymptotic solutions to the problem are proposed for extraction of intrinsic kinetic information.