## Abstract LineweaverβBurk plots of reaction rate data obtained with immobilized enzymes need not be linear even when intrinsic enzyme kinetics follow the simple MichaelisβMenten rate expression. Theoretical calculations show that mass transfer effects may cause curvature which is concave or conve
Diffusional falsification of kinetic constants on Lineweaver-Burk plots
β Scribed by Young Sung Ghim; Ho Nam Chang
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 524 KB
- Volume
- 105
- Category
- Article
- ISSN
- 0022-5193
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π SIMILAR VOLUMES
Statistical methods for the analysis of initial-velocity and/or inhibition data are described. They involve application of F tests (i) to determine goodness of fit to the first-order Michaelis-Menten equation, (ii) to predict the reaction mechanism by assessing slope and y-intercept effects in Linew
## Abstract The effect of internal diffusion on the slope and the intercept of the LineweaverBurk plots for the immobilized enzyme was considered theoretically and it was found that the slope and the intercept are influenced not only by the dimensionless term __M__ but also by the range of the dime