Female Sprague-Dawley rats exhibited significantly improved growth after a 30-day feeding with lysine-supplemented rations. Supplementation with lysine and tryptophan led to improved growth in two of three experimental trials. Although increased weight gain was noted in male rats fed the supplemented rations, the results were significant in only one of three trials. The order of limiting amino acids and the supplementation level were calculated on the basis of the fasting plasma amino acid concentrations. After ethanol (6 g/kg PO), animals fed either supplemented ration showed significant sleeping time reductions and significant increases in the onset of loss of the righting reflex. The ethanol LD50 value increased significantly only for the rats fed the ration supplemented with both amino acids. After pentobarbital (20 mg/kg PO), the onset of loss of the righting reflex increased significantly in rats fed the lysine-tryptophan-supplemented ration. Effects on sleeping time and the LDm were insignificant with rats fed either supplemented ration. Rats fed either supplemented ration showed significant sleeping time decreases and increases in the onset of loss of the righting reflex after hexobarbital (28 mg/kg PO). No significant effect on the hexobarbital LDm was observed with either supplemented ration.
Keyphrases Dietary supplements-effect of lysine and tryptophan on growth rate, barbiturate toxicity, ethanol toxicity, rats Essential amino acids-effect of dietary lysine and tryptophan supplementation on growth rate, barbiturate toxicity, ethanol toxicity 0 Pentobarbital toxicity-effect of dietary lysine and tryptophan supplementation Hexobarbital toxicity-effect of dietary lysine and tryptophan supplementation Ethanol toxicity-effect of dietary lysine and tryptophan supplementation
The ability of rats to tolerate ethanol was shown to vary with the nature of their dietary protein (1). When rats were fed a diet that promoted better growth, they generally became less intoxicated as compared with rats fed a diet that was not as effective in growth promotion. Such reduced intoxication was observed after chronic administration. Among the natural protein mixtures studied (l), egg and milk proteins were more effective than those from cereal and other plant proteins. Milk protein and egg protein were equally effective in protecting the rats against ethanol toxicity. However, milk protein was considerably less effective against the inebriating effects of ethanol.
Rockland Farms mousehat diet. Sigma Chemical Co.