Effect of dexamethasone on herpes simplex virus replication in mouse neuroblastoma cells (NB41 A3): Receptor characteristics

✍ Scribed by Larry L. Dreyer; Dr. Robert J. Sydiskis; Nasir Bashirelahi

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 680 KB
• Volume: 3
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860030408

✦ Synopsis

Monolayers of NB41A3 (MNB) cells were exposed to pharmacologic doses of dexamethasone (DXM). After 24-h, the cells were infected with herpes simplex virus type 1 (HSV-I) at a multiplicity of infection (m.0.i.) = 0.1. After every 24-h period, extracellular and intracellular virus aliquots were collected and frozen. The aliquots were titered in a standard plaque-forming assay. It was shown that the hormone led to a statistically significant increase of extra-and intracellular virus titers above the titers exhibited by these cells without added hormone. The same experiment was repeated in Vero cells,