The effects of agitation on fragmentation of a recombinant strain of Aspergillus oryzae and its consequential effects on protein production have been investigated. Constant mass, 5.3-L chemostat cultures at a dilution rate of 0.05 h -1 and a dissolved oxygen level of 75% air saturation, have been co
Effect of deletion of chitin synthase genes on mycelial morphology and culture viscosity in Aspergillus oryzae
✍ Scribed by Christian Müller; Kim Hansen; Peter Szabo; Jens Nielsen
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 263 KB
- Volume
- 81
- Category
- Article
- ISSN
- 0006-3592
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Highly branched mutants of two strains of Aspergillus oryzae (IFO4177, which produces ␣-amylase, and a transformant of IFO4177 [AMG#13], which produces heterologous glucoamylase in addition to ␣-amylase) were generated by UV or nitrous acid mutagenesis. Four mutants of the parental strain (IFO4177),
## Abstract We previously reported that, although agitation conditions strongly affected mycelial morphology, such changes did not lead to different levels of recombinant protein production in chemostat cultures of __Aspergillus oryzae__ (Amanullah et al., 1999). To extend this finding to another s
The response of steady state chemostat cultures of a recombinant Aspergillus niger (B1-D), secreting both a heterologous enzyme (Hen Egg White Lysozyme [HEWL]) and a native enzyme (Glucoamylase), to varying levels of O 2 enrichment of the process gas was evaluated. Formation of both the native and t