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Effect of cyclodextrin as mobile phase additive on fluorescence intensity of dansylamino acids in microcolumn liquid chromatography

✍ Scribed by Toyohide Takeuchi; Tomoo Miwa


Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
409 KB
Volume
292
Category
Article
ISSN
0003-2670

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✦ Synopsis


The effects of cyclodextrin (CD) as mobile phase additive on the fluorescence intensity of dansylamino acids were investigated in microcolumn liquid chromatography. Signal enhancement was observed for /3-and y-CD, whereas no signal enhancement was observed for a-CD. The signal enhancement was pronounced for neutral dansylamino acids, and larger fluorescence enhancement was observed for mobile phases containing higher concentrations of CD.


📜 SIMILAR VOLUMES


Enantiomeric resolution of dansyl amino
✍ Toyohide Takeuchi 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 415 KB 👁 1 views

## Abstract Enantiomeric resolution of dansyl amino acids by microcolumn liquid chromatography with γ‐cyclodextrin as a mobile phase additive has been investigated. The separation factor for the enantiomers increased with increasing γ‐cyclodextrin concentration as well as with decreasing acetonitri

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✍ Toyohide Takeuchi; Tomoo Miwa; Jianhua Chu 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 471 KB

## Abstract The effects of γ‐cyclodextrin and bovine serum albumin as mobile phase additives on fluorescence intensity and retention of dansyl amino acids (Dns‐AAs) have been examined in microcolumn liquid chromatography. Polymercoated mixed‐functional silica packings were employed for the separati

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✍ Takeuchi, Toyohide ;Nagae, Norikazu 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 236 KB 👁 1 views

## Abstract Dansyl derivatives of racemic phenylalanine and its analogs have been resolved by microcolumn liquid chromatography with γ‐cyclodextrin as mobile phase additive. The enantioselectivity of the system was influenced by the concentrations of both γ‐cyclodextrin and acetonitrile in the mobi