Effect of column performance on the accuracy of molecular weights obtained from size exclusion chromatography (gel permeation chromatography)
β Scribed by W.W. Yau; J.J. Kirkland; D.D. Bly; H.J. Stoklosa
- Book ID
- 108335696
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 841 KB
- Volume
- 125
- Category
- Article
- ISSN
- 1873-3778
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π SIMILAR VOLUMES
A high-performance size-exclusion chromatographic system is described that minimizes the ionic and hydrophobic interactions of proteins with the stationary phase. The system can be used to determine reliably the molecular weight of proteins between M, 10,000 and 70,000. A further application would b
Gel permeation chromatography produces a type of differential molecular weight distribution directly and rapidly. Conversion of these data to conventional molecular weight distributions and plots of distributions is time-consuming. A computer program is described to perform these operations readily.