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Effect of Circularity and Superhelicity on Transcription from Bacteriophage λ DNA

✍ Scribed by Peter Botchan, James C. Wang and Harrison Echols

Book ID
123651912
Publisher
National Academy of Sciences
Year
1973
Tongue
English
Weight
876 KB
Volume
70
Category
Article
ISSN
0027-8424

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Restriction fragments containing either leut (a rho-independent transcription termination site) and/or leut' (a rho-dependent transcription termination site) were cloned into plasmid pOL4. Treatment of plasmid-containing Escherichia coli strains with coumermycin resulted in loss of in vivo plasmid s