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Effect of DNA superhelicity on transcription termination

โœ Scribed by Rosenthal, Eugene R. ;Calvo, Joseph M.

Book ID
104725826
Publisher
Springer
Year
1987
Tongue
English
Weight
545 KB
Volume
207
Category
Article
ISSN
0026-8925

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โœฆ Synopsis

Restriction fragments containing either leut (a rho-independent transcription termination site) and/or leut' (a rho-dependent transcription termination site) were cloned into plasmid pOL4. Treatment of plasmid-containing Escherichia coli strains with coumermycin resulted in loss of in vivo plasmid superhelicity 10 min after antibiotic addition. Galactokinase levels specified by these plasmid-containing strains were the same regardless of whether functional DNA gyrase was present. These results suggest that transcription termination is unaffected by the superhelical state of DNA.

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