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Effect of chromatographic conditions on resolution in high-performance ion-exchange chromatography of proteins on nonporous support

โœ Scribed by Yoshio Kato; Koji Nakamura; Takashi Kitamura; Teruhiko Tsuda; Masazumi Hasegawa; Hiroo Sasaki

Book ID
108339357
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
134 KB
Volume
1009
Category
Article
ISSN
1873-3778

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Separation of DNA fragments by high-reso
Separation of DNA fragments by high-resolution ion-exchange chromatography on a nonporous QA column
โœ Yoshihiro Ohmiya; Yoichi Kondo; Toshihiko Kondo ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 925 KB

A nonporous QA column (a strong anion exchanger) was used for HPLC of DNA fragments. This column was successfully employed to separate small (ca. 10 bp) and intermediate size (ca. 10 kbp) DNA fragments from each other. The column also separated double-stranded DNA from its single-stranded form, and