Effect of chemical modification of isoenzymes A and B fromC. rugosaon their activity and stability

✍ Scribed by María V. Calvo; Francisco J. Plou; Eitel Pastor; Antonio Ballesteros

• Book ID: 104632818
• Publisher: Springer Netherlands
• Year: 1995
• Tongue: English
• Weight: 353 KB
• Volume: 17
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00127983

✦ Synopsis

Lipases A and B from CJugosa have been chemically modified with octanoyl chloride or polyethylene glycol (molecular weight: 750 or 5000). Although the hydrolytic activity was diminished, modification gave rise in some cases to preparations with increased synthetic activity in hexane. Furthermore, PEGsoco-lipases A and B were significantly more stable (up to 20-fold)at alkaline pH values than the native enzymes.