Effect of cell concentration on the uptake of amino acids by rat liver parenchymal cells in suspension
β Scribed by P. M. Bhargava; M. A. Siddiqui; G. Kranti Kumar; K. S. N. Prasad
- Publisher
- Springer
- Year
- 1975
- Tongue
- English
- Weight
- 638 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0022-2631
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Amino acid transport was studied in primary cultures of parenchymal cells isolated from adult rat liver by a collagenase perfusion technique and maintained as a monolayer in a serum-free culture medium. These cells carried out gluconeogenesis from three carbon precursors (alanine, pyruvate, and lact
The uptake of [45Ca] has been studied in clonal glial and neuronal cells. It was somewhat more efficient in the neuroblastoma clone MI compared to glial clones. In all cases [45Ca] uptake was shown to depend on the phosphate concentration in the incubation medium. It was decreased by the ionophore A
## Abstract Parenchymal cells from adult rat liver, isolated by a collagenase perfusion technique, have been maintained in primary culture and a detailed study on carbohydrate metabolism carried out over the initial 48βhour culture period. The glucose concentration of the medium exerts a major infl