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Effect of buffer constituents on rat liver 3-hydroxy-3-methyl glutaryl coenzyme A reductase

โœ Scribed by M. V. Srikantaiah; N. Noble; L. Orenstein; R. J. Morin


Book ID
112773166
Publisher
Springer-Verlag
Year
1981
Tongue
English
Weight
172 KB
Volume
16
Category
Article
ISSN
0024-4201

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A rapid method for the purification of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase from the livers of cholestyramine-fed rats is reported. The procedure involves a sequence of separations on affinity chromatography columns consisting of Blue Dextran-Sepharose. agarose-CoA, and agarose-