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Rapid, high-yield purification of rat liver 3-hydroxy-3-methyl-glutaryl-coenzyme a reductase

✍ Scribed by David H. Rogers; S.R. Panini; Harry Rudney

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
401 KB
Volume
101
Category
Article
ISSN
0003-2697

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✦ Synopsis

A rapid method for the purification of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase from the livers of cholestyramine-fed rats is reported. The procedure involves a sequence of separations on affinity chromatography columns consisting of Blue Dextran-Sepharose. agarose-CoA, and agarose-HMG-CoA. The advantage of this method is its flexibility in scavenging enzyme that might be lost during purification, resulting in a yield of homogeneous reductase (specific activity approximately 10,000 nmoliminlmg protein) as high as 50%. which is at least twice that previously reported.

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