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Effect of ascorbic acid on the synaptosomal uptake of [3H]MPP+, [3H]Dopamine, and [14C]GABA

✍ Scribed by Dr. H. Sershen; E. A. Debler; A. Lajtha

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
393 KB
Volume
17
Category
Article
ISSN
0360-4012

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✦ Synopsis

The effects of ascorbic acid on the synaptosomal uptake of r3H]MPP+, [3H]dopamine, and [I4C]GABA were examined in attempts to understand the mechanism of ascorbic acid attenuation of MPTP neurotoxicity. [3HJDopamine uptake was increased at lower levels (0.01 and 0.1 mM) and decreased at higher levels (10 mM) of ascorbic acid. Ascorbic acid inhibited r3H]MPP+ uptake (ICS0 = 0.1 mM) and [I4C]GABA uptake (ICsO = 10 mM). Washout of ascorbic acid restored uptake of [3HJdopamine and [3H]MPP+, suggesting that ascorbic-acid-induced lipid peroxidation was not involved in the effect on uptake. In addition to the possible involvement of antioxidant mechanisms in the in vivo attenuation of the neurotoxicity of MPTP by ascorbic acid, the present results indicate a direct effect of ascorbic acid in inhibiting the uptake of MPP+ into dopaminergic nerve terminals.

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