Effect of antioxidants on chemiluminescence produced by reactive oxygen species

✍ Scribed by Pascual, Carlos ;Romay, Cheyla

• Publisher: John Wiley and Sons
• Year: 1992
• Weight: 680 KB
• Volume: 7
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170070205

✦ Synopsis

Luminol chemiluminescence was used t o evaluate the scavenging of superoxide, hydroxyl and alkoxy radicals by four antioxidants: dipyridamole, diethyldithiocarbamic acid, ( + )catechin, and ascorbic acid. Different concentrations of these compounds were compared w i t h well-known oxygen radical scavengers in their capacity t o inhibit the chemiluminescence produced in the reaction between luminol and specific oxygen radicals.

Hydroxyl radicals were generated using the Fenton reaction and these produced chemiluminescence which was inhibited by diethyldithiocarbamate. Alkoxy radicals were generated using the reaction of tert-butyl hydroperoxide and ferrous ion and produced chemiluminescence which was inhibited equally by all of the compounds tested. For the determination of superoxide scavengers w e describe a new. simple, economic, and rapid chemiluminescence method consisting of the reaction between luminol and horseradish peroxidase (HRP). With this method it was found that 40 nmol/l dipyridamole, 0.18 pmol/l ascorbic acid, 0.23 pmol/l (+)catechin, and 3 pmol/l diethyldithiocarbamic acid are equivalent t o 3.9 ng/ml superoxide dismutase (specific scavenger of superoxide) in causing the same degree of chemiluminescence inhibition.

These results not only indicated that the antioxidative properties of these compounds showed different degrees o f effectiveness against a particular radical but also that they may exert their action against more than one radical.