Effect of amiodarone (AMD) on the antioxidant enzymes, lipid peroxidation and mitochondrial metabolism

✍ Scribed by Sonia Machado R. Ribeiro; Annibal P. Campello; Aguinaldo J. Nascimento; Ma. Lúcia W. Kluppel

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 151 KB
• Volume: 15
• Category: Article
• ISSN: 0263-6484
• DOI: 10.1002/(sici)1099-0844(199709)15:3<145::aid-cbf728>3.0.co;2-x

✦ Synopsis

The eects of amiodarone (AMD) on lipid peroxidation of rat liver mitochondria, the formation of superoxide anions at the respiratory chain level, and the cytosolic and mitochondrial enzymatic protective mechanisms of oxidative stress were studied. An attempt of classify AMD according to its toxic ability to interfere with the integrated function of electron transport enzymes was also investigated. The results con®rm the eects of AMD on complex I and permit the placing of this drug in class A of the classi®cation of Knobeloch, together with rotenone, amytal and chaotropic agents. AMD has no eect on the activity of the enzymes superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase, nor on glucose 6-phosphate dehydrogenase. AMD did not promote an increase in the formation of anion superoxide at the respiratory chain level. Pre-incubation with AMD (16Á6 mM) inhibited about 70 per cent of lipid peroxidation. The results suggest a protective eect of AMD against lipid peroxidation in mitochondrial membranes by iron-dependent systems.