Effect of alcohols and temperature on the direct chiral resolutions of fipronil, isocarbophos and carfentrazone-ethyl

✍ Scribed by Peng Wang; Shuren Jiang; Donghui Liu; Guifang Jia; Qiuxia Wang; Ping Wang; Zhiqiang Zhou

• Book ID: 101760371
• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 86 KB
• Volume: 19
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.505

✦ Synopsis

Abstract

The enantiomeric separations of three pesticides fipronil (asymmetric nitrogen), isocarbophos (asymmetric phosphorus) and carfentrazone‐ethyl (asymmetric carbon) were studied on cellulose‐tri(3,5‐dimethylphenylcarbamate) chiral stationary phase using high‐performance liquid chromatography under normal phase. The mobile phase was n‐hexane with alcohols including ethanol, n‐propanol, iso‐propanol, n‐butanol and iso‐butanol as polar modifiers. The flow rate was 1.0 mL/min with UV detection at 280, 225 and 230 nm for fipronil, isocarbophos and carfentrazone‐ethyl respectively. The influence of the modifiers and their volume content and temperature from 0 to 50°C on the separations was investigated. The chiral stationary phase showed excellent stereoselectivity for the two enantiomers of fipronil and isocarbophos and certain chiral recognition for carfentrazone‐ethyl. Iso‐propanol was more suitable for the chiral separation of isocarbophos and carfentrazone‐ethyl, and iso‐butanol was better for fipronil. The resolutions increased with the decreasing modifier content and temperature for all the three chiral pesticides. Copyright © 2005 John Wiley & Sons, Ltd.