Effect of 2,3-butanedione monoxime on force of contraction and protein phosphorylation in bovine smooth muscle
✍ Scribed by R. Waurick; J. Knapp; H. Van Aken; P. Bokník; J. Neumann; W. Schmitz
- Publisher
- Springer-Verlag
- Year
- 1999
- Tongue
- English
- Weight
- 171 KB
- Volume
- 359
- Category
- Article
- ISSN
- 0028-1298
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The effects of 2,3-butanedione monoxime (BDM) were studied in smooth muscle fibres from guinea pig taenia colt. In intact muscle, active force during contractions induced by high-K + was inhibited by about 10% in I mM BDM and by approximately 70% in 10 mM BDM. Intracellular [Ca 2+ ] during contracti
2,3-butanedione 2-monoxime (BDM) inhibits muscle contraction and actomyosin ATPase both in fibres and in solution. It is potentially useful as a tool for exploring weak interactions between actin and myosin. We have examined the effect of BDM on several key steps of the myosin subfragment-1 and acto