Effect of 2,2,2-trifluoroethanol on capillary zone electrophoretic peptide separations
β Scribed by Massimo Castagnola; Loredana Cassiano; Irene Messana; Maurizio Paci; Diana Valeria Rossetti; Bruno Giardina
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 691 KB
- Volume
- 735
- Category
- Article
- ISSN
- 1873-3778
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π SIMILAR VOLUMES
A series of 10 synthetic peptides containing varying degrees of charge and hydrophobicity was used to study the effects of peptide composition and buffer pH on the selectivity of separations by capillary zone electrophoresis (CZE). A simple model is used to explain the effect of buffer pH on the sep
## Abstract Binding of Zn^2+^ to bacitracin A~1~ was studied by capillary electrophoresis in water/2,2,2βtrifluoroethanol (70/30 v/v) at different apparent pH values in order to estimate the association constant of metal, the acidic dissociation constants and the Stokes radii of both free and bound
It was found that unlike the electroosmotic flow, the electrophoretic mobilities of small peptides in capillary zone electrophoresis (CZE) were invariant over the buffer concentration range studied. Migration of these peptides in CZE is a electroosmotic-governed process. The quantitative linear rela