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Editing of Multidimensional NMR Spectra of Partially Deuterated Proteins. Measurement of Amide Deuterium Isotope Effects on the Chemical Shifts of Protein Backbone Nuclei

✍ Scribed by Axel Meissner; Jacques Briand; Ole Winneche Sφrensen


Book ID
110260853
Publisher
Springer Netherlands
Year
1998
Tongue
English
Weight
194 KB
Volume
12
Category
Article
ISSN
0925-2738

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New Multidimensional Editing Experiments
✍ Axel Meissner; Ole Winneche Sørensen 📂 Article 📅 1998 🏛 Elsevier Science 🌐 English ⚖ 131 KB

Novel multidimensional NMR pulse sequences for measurement of the three- and four-bond amide deuterium isotope effect on the chemical shifts of 13Cbeta in proteins are presented. The sequences result in editing into two subspectra of a heteronuclear triple resonance spectrum ¿omega(N), omega(Cbeta),