New Multidimensional Editing Experiments for Measurement of Amide Deuterium Isotope Effects on CβChemical Shifts in13C,15N-Labeled Proteins

✍ Scribed by Axel Meissner; Ole Winneche Sørensen

• Publisher: Elsevier Science
• Year: 1998
• Tongue: English
• Weight: 131 KB
• Volume: 135
• Category: Article
• ISSN: 1090-7807
• DOI: 10.1006/jmre.1998.1567

✦ Synopsis

Novel multidimensional NMR pulse sequences for measurement of the three- and four-bond amide deuterium isotope effect on the chemical shifts of 13Cbeta in proteins are presented. The sequences result in editing into two subspectra of a heteronuclear triple resonance spectrum ¿omega(N), omega(Cbeta), omega(Halpha)¿ according to there being a deuterium or a proton attached to 15N for the pertinent correlations. The new experiments are demonstrated by an application to the first module of the 13C,15N-labeled protein RAP 18-112 (N-terminal module of alpha2-macroglobulin receptor associated protein).