Ectopic expression of p27Kip1 in oligodendrocyte progenitor cells results in cell-cycle growth arrest

✍ Scribed by Tikoo, Ravi ;Osterhout, Donna J. ;Casaccia-Bonnefil, Patrizia ;Seth, Prem ;Koff, Andrew ;Chao, Moses V.

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 340 KB
• Volume: 36
• Category: Article
• ISSN: 0022-3034
• DOI: 10.1002/(sici)1097-4695(19980905)36:3<431::aid-neu10>3.0.co;2-e

✦ Synopsis

Oligodendrocyte differentiation is a ble for G1/S transition, as immunodepletion of oligocomplex process believed to be controlled by an intrindendrocyte extracts of p27 protein resulted in the actisic mechanism associated with cell-cycle arrest. Revation of CDK2 activity. However, cells that became cently, the cell-cycle inhibitor protein p27 Kip1 has been growth arrested owing to infection with p27 adenoviproposed as a key element in causing growth arrest rus did not display conventional oligodendrocyte of oligodendrocyte precursor cells. To investigate the differentiation markers, such as O4 or O1. Taken toeffects of p27 upon oligodendrocyte cell development, gether, these data provide mechanistic evidence indiwe have introduced the p27 cDNA in oligodendrocyte cating that p27 is primarily involved in oligodendrogprogenitor cells using an adenovirus vector. Progenilial progenitor proliferation by inhibiting CDK2 activtor cells normally express low levels of p27. After adeity and inducing oligodendrocyte cell-cycle arrest. noviral infection and p27 overexpression, progenitor