EcoRI restriction-site polymorphism of the albumin gene in different inbred strains of rat

✍ Scribed by Gérard Lucotte; Andras Gal; Jean-Louis Nahon; José M. Sala-Trepat

• Publisher: Springer
• Year: 1982
• Tongue: English
• Weight: 539 KB
• Volume: 20
• Category: Article
• ISSN: 0006-2928
• DOI: 10.1007/bf00498935

✦ Synopsis

Two types of variant EcoRI restriction enzyme patterns of albumin-gene DNA fragments have been detected in different rat strains by agarose gel electrophoresis and Southern blot hybridization using 32P-labeled cloned rat albumin cDNA probes. The type I albumin gene variant is characteristic of the Sprague-Dawley strain, and type II is found in Buffalo rats. The occurrence of these variants is interpreted as the result of simple allelic polymorphism because they are inherited in a normal Mendelian fashion when crossing Sprague-Dawley and Buffalo rats. The distribution of the two genetic variants in various inbred strains of rat suggests that type I represents the original or ancestral form of the albumin gene and that type II appeared spontaneously during laboratory breeding.